baa 1605 pmrab mutant strains Search Results


baa 1605  (ATCC)
97
ATCC baa 1605
Frequency of emergence of colistin-resistant A. baumannii (ATCC <t>BAA-1605)</t> strains and change in mutation tendency in combination with meropenem. (A) The frequency of colistin resistance was analyzed by plating 0.1 mL culture (OD 600 1.0) on LB agar containing 5× MIC (10 μg/mL) of colistin for 24 h at 37°C. The frequency of colistin resistance in combination with 1/5× MIC (2 μg/mL) of meropenem was determined by plating 0.1 mL culture (OD 600 of 25) on LB agar containing 10 μg/mL of colistin and 2 μg/mL of meropenem. Data are shown as a box plot; 20 independent assays were compared by the Mann-Whitney U test. ***, P < 0.001. (B) Mutation site analysis of colistin- and colistin-meropenem-resistant ATCC BAA-1605 strains. Sanger sequencing of the genomic regions containing the pmrAB genes was performed. Mutations in colistin-resistant strains and colistin-meropenem-resistant strains are shown above and below the genes, respectively. In the boxes, base changes are shown on top, while amino acid changes are shown below. The numerical values are the numbers of isolated strains, and numbers in parentheses are the numbers of independent events.
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Frequency of emergence of colistin-resistant A. baumannii (ATCC BAA-1605) strains and change in mutation tendency in combination with meropenem. (A) The frequency of colistin resistance was analyzed by plating 0.1 mL culture (OD 600 1.0) on LB agar containing 5× MIC (10 μg/mL) of colistin for 24 h at 37°C. The frequency of colistin resistance in combination with 1/5× MIC (2 μg/mL) of meropenem was determined by plating 0.1 mL culture (OD 600 of 25) on LB agar containing 10 μg/mL of colistin and 2 μg/mL of meropenem. Data are shown as a box plot; 20 independent assays were compared by the Mann-Whitney U test. ***, P < 0.001. (B) Mutation site analysis of colistin- and colistin-meropenem-resistant ATCC BAA-1605 strains. Sanger sequencing of the genomic regions containing the pmrAB genes was performed. Mutations in colistin-resistant strains and colistin-meropenem-resistant strains are shown above and below the genes, respectively. In the boxes, base changes are shown on top, while amino acid changes are shown below. The numerical values are the numbers of isolated strains, and numbers in parentheses are the numbers of independent events.

Journal: Microbiology Spectrum

Article Title: Preferential Selection of Low-Frequency, Lipopolysaccharide-Modified, Colistin-Resistant Mutants with a Combination of Antimicrobials in Acinetobacter baumannii

doi: 10.1128/spectrum.01928-22

Figure Lengend Snippet: Frequency of emergence of colistin-resistant A. baumannii (ATCC BAA-1605) strains and change in mutation tendency in combination with meropenem. (A) The frequency of colistin resistance was analyzed by plating 0.1 mL culture (OD 600 1.0) on LB agar containing 5× MIC (10 μg/mL) of colistin for 24 h at 37°C. The frequency of colistin resistance in combination with 1/5× MIC (2 μg/mL) of meropenem was determined by plating 0.1 mL culture (OD 600 of 25) on LB agar containing 10 μg/mL of colistin and 2 μg/mL of meropenem. Data are shown as a box plot; 20 independent assays were compared by the Mann-Whitney U test. ***, P < 0.001. (B) Mutation site analysis of colistin- and colistin-meropenem-resistant ATCC BAA-1605 strains. Sanger sequencing of the genomic regions containing the pmrAB genes was performed. Mutations in colistin-resistant strains and colistin-meropenem-resistant strains are shown above and below the genes, respectively. In the boxes, base changes are shown on top, while amino acid changes are shown below. The numerical values are the numbers of isolated strains, and numbers in parentheses are the numbers of independent events.

Article Snippet: By confirming isogenic pmrAB mutants, we succeeded in producing mutation libraries in the background of two A. baumannii strains, ATCC 19606 and ATCC BAA-1605, and present useful data for understanding the colistin resistance mechanism and the PmrAB two-component system.

Techniques: Mutagenesis, MANN-WHITNEY, Sequencing, Isolation